A validated bioluminescence - based assay for the rapid determination of the initial rate of 1 kill for discovery

نویسندگان

  • Imran Ullah
  • Raman Sharma
  • Giancarlo A. Biagini
  • Paul Horrocks
چکیده

16 Objectives: A future treatment for uncomplicated malaria will contain at least one 17 component that exerts a rapid rate of kill (RoK). We describe here the validation and 18 application of a simple, robust and rapid bioluminescence-based assay for the 19 determination of the initial RoK in intraerythrocytic asexual stages of Plasmodium 20

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A validated bioluminescence-based assay for the rapid determination of the initial rate of kill for discovery antimalarials.

Objectives A future treatment for uncomplicated malaria will contain at least one component that exerts a rapid rate of kill. We describe here the validation and application of a simple, robust and rapid bioluminescence-based assay for the determination of the initial rate of kill in intra-erythrocytic asexual stages of Plasmodium falciparum . Methods A modification to the concentration-respo...

متن کامل

Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma

A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150?4 mm) and a UV detector (?= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...

متن کامل

Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma

A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150?4 mm) and a UV detector (?= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...

متن کامل

Selective and Validated SpectrophotometricMethods for Determination of Acyclovir and Ganciclovirwith 2, 4 as Reagent

New, simple and sensitive spectrophotometric methods for the determination of acyclovir and ganciclovirhave been developed. The method is based on the oxidation of 2, 4-dinitrophenyl hydrazine (2, 4 DNP) and coupling of the oxidized product with acyclovir and ganciclovirto give intensely coloredchromogen. Acyclovir and ganciclovirshowed maximum absorbance at 414 nm and 450 nm with linearity was...

متن کامل

Toward the Development of a Single-Round Infection Assay Based on EGFP Reporting for Anti-HIV-1 Drug Discovery

Background: The rapid increase of HIV-1 strains resistant to current antiretroviral drugs is a challenge for successful AIDS therapy. This necessitates the development of novel drugs, and to this end, availability of screening systems for in vitro drug discovery is a priority. Herein, we report the modification of a previously developed system for increased sensitivity, ease of use, and cost-ef...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2016